PerlPrimer
PerlPrimer is a GUI application that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR), and sequencing. It aims to automate and simplify the process of primer design. Current features include calculation of possible primer-dimers, retrieval of genomic or CDNA sequences from Ensembl (including both sequences automatically for QPCR), the ability to BLAST search primers using the NCBI server, ORF, and CpG island detection algorithms, the ability to add cloning sequences to primers, automatically adjusted to be in-frame, and QPCR primer design without manual intron-exon boundary entry.
| Tags | Scientific/Engineering Bioinformatics |
|---|---|
| Licenses | GPL |
| Operating Systems | OS Independent |
| Implementation | Perl |
Recent releases
- 18 Oct 2006 07:37
Changes: This release again restores compatibility with the Ensembl online database.
- 05 Jun 2006 03:57
Changes: This release fixes compatibility with the Ensembl database, and enables the retrieval of unannotated genes from Ensembl (using the Ensembl Gene ID). Compatibility with NCBI BLAST search results was restored. There's also an update to the ORF search routines and a minor bugfix.
- 20 Mar 2006 03:37
Changes: This release once again restores compatibility with the Ensembl database, adds a button to view the alternative transcripts when retrieving a gene from Ensembl, updates the list of Ensembl genome databases, and adds several minor cosmetic enhancements to the GUI.
- 09 Sep 2005 01:48
Changes: This release once again fixes compatibility with the Ensembl databases.
- 09 Aug 2005 03:20
Changes: This release restores compatibility with the Ensembl databases and adds an indication of sequence length to the "View intron/exon structure" window for real-time PCR.
- All comments
Recent commentsRe: Wow.
> Even the guys in my Applications unit
> have no idea what this program
> description means. Of course, we've
> downloaded it and will see if we can
> work it out... : )
*grin* It's bioinformatics software for molecular biology - PCR stands for the Polymerase Chain Reaction, and allows you to amplify DNA. It's a pretty fundamental technique that's used in everything from clinical diagnosis to pure research.
But the program's not going to be very useful unless you're a molecular biologist!
Wow.
Even the guys in my Applications unit have no idea what this program description means. Of course, we've downloaded it and will see if we can work it out... : )